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Merck & Co anti flag m2 magnetic beads
Anti Flag M2 Magnetic Beads, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+flag+m2+magnetic+beads/anti+flag+%CE%B1/pm41932879-132-39-43
Average 86 stars, based on 1 article reviews
anti flag m2 magnetic beads - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Magnetic Beads:

Article Title: Fully Tunable Phosphorylation of RPS6A Ensures the Successful Development of Arabidopsis Seedlings
Article Snippet: .. The supernatant was mixed with ANTI‐FLAG M2 magnetic beads (Merck) for 16 h at 4°C. ..

Article Title: TagF coordinates spike-loading as an intermediate checkpoint in Type VI Secretion System assembly
Article Snippet: .. Equilibrated ANTI-FLAG® M2 magnetic beads (Merck) were loaded with 1 ml of clarified cell lysate. ..

Article Title: PARP16 protects against cardiac hypertrophic response by ADP-ribosylation-dependent inhibition of NFAT transcription factor
Article Snippet: .. 1000 μg protein was incubated with 15 μL of Anti-FLAG® M2 Magnetic Beads (Merck) for 4 hours at 4°C with gentle rotation. .. Bead-bound proteins were washed three times with ice-cold TBS and eluted by adding 2× Laemmli sample buffer (Bio-Rad) followed by boiling at 96°C for 5 minutes.

Article Title: Activator–promoter compatibility in mammals: a CpG-Island-specific co-activator directly bridges transcription factors to TFIID
Article Snippet: .. Supernatants were incubated with Anti-FLAG M2 Magnetic Beads (Merck), pre-equilibrated in NET buffer, overnight at 4 °C with rotation. ..

Article Title: Lactic acid drives NLRP3 inflammasome activation and caspase-1-like cytokine cleavage via intracellular acidification.
Article Snippet: The samples were then precipitated with PureProteomeTM Protein G Magnetic Bead (Merck, #LSKMAGG10) for 1 h at 4 °C. .. For purification of pro-IL-1β-FLAG protein, cell extracts from HEK293T cells transfected with pLAS2w.Pneo-pro-IL-1β-FLAG plasmids expressing either wild-type (WT) or Asp116 mutants (D116A, D116N) of pro-IL-1β—obtained from the National RNAi Core Facility, Academia Sinica (Taipei, Taiwan)—were subjected to immunoprecipitation using anti-FLAG® M2 Magnetic Beads (Merck, #M8823) overnight at 4 °C. ..

Article Title: bZIP factors of the Unfolded Protein Response interact with PIF4 to promote thermomorphogenesis
Article Snippet: .. The supernatant was mixed with 40 μl anti-myc magnetic beads (for bZIP28N and PIF4 interaction test) (Santa Cruz, sc-500772) or anti-flag M2 magnetic beads (Merck, Cat no. M8823) for 4 h at 4°. ..

Article Title: Fully Tunable Phosphorylation of RPS6A Ensures the Successful Development of Arabidopsis Seedlings.
Article Snippet: .. The supernatant was mixed with ANTI‐FLAG M2 magnetic beads (Merck) for 16 h at 4°C. ..

Article Title: bZIP factors of the Unfolded Protein Response interact with PIF4 to promote thermomorphogenesis.
Article Snippet: .. The supernatant was mixed with 40μl anti-myc magnetic beads (for bZIP28N and PIF4 interaction test) (Santa Cruz, sc-500772) or anti-flag M2 magnetic beads (Merck, Cat no. M8823) for 4 h at 4°. ..

Incubation:

Article Title: PARP16 protects against cardiac hypertrophic response by ADP-ribosylation-dependent inhibition of NFAT transcription factor
Article Snippet: .. 1000 μg protein was incubated with 15 μL of Anti-FLAG® M2 Magnetic Beads (Merck) for 4 hours at 4°C with gentle rotation. .. Bead-bound proteins were washed three times with ice-cold TBS and eluted by adding 2× Laemmli sample buffer (Bio-Rad) followed by boiling at 96°C for 5 minutes.

Article Title: Activator–promoter compatibility in mammals: a CpG-Island-specific co-activator directly bridges transcription factors to TFIID
Article Snippet: .. Supernatants were incubated with Anti-FLAG M2 Magnetic Beads (Merck), pre-equilibrated in NET buffer, overnight at 4 °C with rotation. ..

Gentle:

Article Title: PARP16 protects against cardiac hypertrophic response by ADP-ribosylation-dependent inhibition of NFAT transcription factor
Article Snippet: .. 1000 μg protein was incubated with 15 μL of Anti-FLAG® M2 Magnetic Beads (Merck) for 4 hours at 4°C with gentle rotation. .. Bead-bound proteins were washed three times with ice-cold TBS and eluted by adding 2× Laemmli sample buffer (Bio-Rad) followed by boiling at 96°C for 5 minutes.

Purification:

Article Title: Lactic acid drives NLRP3 inflammasome activation and caspase-1-like cytokine cleavage via intracellular acidification.
Article Snippet: The samples were then precipitated with PureProteomeTM Protein G Magnetic Bead (Merck, #LSKMAGG10) for 1 h at 4 °C. .. For purification of pro-IL-1β-FLAG protein, cell extracts from HEK293T cells transfected with pLAS2w.Pneo-pro-IL-1β-FLAG plasmids expressing either wild-type (WT) or Asp116 mutants (D116A, D116N) of pro-IL-1β—obtained from the National RNAi Core Facility, Academia Sinica (Taipei, Taiwan)—were subjected to immunoprecipitation using anti-FLAG® M2 Magnetic Beads (Merck, #M8823) overnight at 4 °C. ..

Transfection:

Article Title: Lactic acid drives NLRP3 inflammasome activation and caspase-1-like cytokine cleavage via intracellular acidification.
Article Snippet: The samples were then precipitated with PureProteomeTM Protein G Magnetic Bead (Merck, #LSKMAGG10) for 1 h at 4 °C. .. For purification of pro-IL-1β-FLAG protein, cell extracts from HEK293T cells transfected with pLAS2w.Pneo-pro-IL-1β-FLAG plasmids expressing either wild-type (WT) or Asp116 mutants (D116A, D116N) of pro-IL-1β—obtained from the National RNAi Core Facility, Academia Sinica (Taipei, Taiwan)—were subjected to immunoprecipitation using anti-FLAG® M2 Magnetic Beads (Merck, #M8823) overnight at 4 °C. ..

Expressing:

Article Title: Lactic acid drives NLRP3 inflammasome activation and caspase-1-like cytokine cleavage via intracellular acidification.
Article Snippet: The samples were then precipitated with PureProteomeTM Protein G Magnetic Bead (Merck, #LSKMAGG10) for 1 h at 4 °C. .. For purification of pro-IL-1β-FLAG protein, cell extracts from HEK293T cells transfected with pLAS2w.Pneo-pro-IL-1β-FLAG plasmids expressing either wild-type (WT) or Asp116 mutants (D116A, D116N) of pro-IL-1β—obtained from the National RNAi Core Facility, Academia Sinica (Taipei, Taiwan)—were subjected to immunoprecipitation using anti-FLAG® M2 Magnetic Beads (Merck, #M8823) overnight at 4 °C. ..

Immunoprecipitation:

Article Title: Lactic acid drives NLRP3 inflammasome activation and caspase-1-like cytokine cleavage via intracellular acidification.
Article Snippet: The samples were then precipitated with PureProteomeTM Protein G Magnetic Bead (Merck, #LSKMAGG10) for 1 h at 4 °C. .. For purification of pro-IL-1β-FLAG protein, cell extracts from HEK293T cells transfected with pLAS2w.Pneo-pro-IL-1β-FLAG plasmids expressing either wild-type (WT) or Asp116 mutants (D116A, D116N) of pro-IL-1β—obtained from the National RNAi Core Facility, Academia Sinica (Taipei, Taiwan)—were subjected to immunoprecipitation using anti-FLAG® M2 Magnetic Beads (Merck, #M8823) overnight at 4 °C. ..



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